The journal club is intended to be a weekly natter about interesting recent research. We tend to stick to high-impact journals - Nature, Science, PNAS and PRL have been popular - but this is not prescriptive. Given the diversity of research in the CM group, chosen topics vary widely. Anyone and everyone is welcome: if you have a paper you want to discuss, email it to me (Joe Tavacoli
) and I'll slot you in.
Week beginning 31 January 2016
Monday 1 Feb 16 - 11:30am
To be confirmed
Friday 5 Feb 16 - 11:30am
A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
Authors: Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauser, Jennifer A. Doudna, Emmanuelle Charpentier
Speaker: Delma Childers
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. We show here that in a subset of these systems, the mature crRNA that is base-paired to trans-activating crRNA (tracrRNA) forms a two-RNA structure that directs the CRISPR-associated protein Cas9 to introduce double-stranded (ds) breaks in target DNA. At sites complementary to the crRNA-guide sequence, the Cas9 HNH nuclease domain cleaves the complementary strand, whereas the Cas9 RuvC-like domain cleaves the noncomplementary strand. The dual-tracrRNA:crRNA, when engineered as a single RNA chimera, also directs sequence-specific Cas9 dsDNA cleavage. Our study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
816-821 (2012) pdf version Supplimentary Information
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