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Experimentally manipulating fungi with optical tweezers [1%] by admin, 2007-07-09 09:34
A short review of the use of optical tweezers in fungal cell biological research is provided. First, we describe how optical tweezers work. Second, we review ...
High-speed holographic optical tweezers using a ferroelectric liquid crystal microdisplay [1%] by admin, 2007-07-06 16:43
We demonstrate the advantages of a ferroelectric liquid crystal spatial light modulator for optical tweezer array applications. The ...
Time-resolved FRET and FLIM of four-way DNA junctions [1%] by admin, 2007-07-06 16:42
Conformational transitions in a 4-way DNA junction when titrated with ionic solutions are studied using time-resolved fluorescence ...
The stability and characteristics of a DNA holliday junction switch [1%] by admin, 2007-07-06 16:41
A Holliday junction (HJ) consists of four DNA double helices, with a branch point discontinuity at the intersection of the component ...
Improved silicon nitride surfaces for next-generation microarrays [1%] by admin, 2007-07-06 16:40
This work reports how the use of a standard integrated circuit (IC) fabrication process can improve the potential of silicon nitride ...
Electrochemical deposition of Zn on TiN microelectrode arrays for microanodes [1%] by admin, 2007-07-09 09:19
Zn was electrochemically deposited onto square TiN electrodes with edge
Probing the liquid-state structure and dynamics of aqueous solutions by fluorescence spectroscopy [1%] by admin, 2007-07-06 16:35
Time-resolved fluorescence spectroscopy of the solvent- sensitive molecule 1,8-anilinonaphthalene sulfonate (ANS) is used to probe ...
Evidence of tautomerism in 2-aminopurine from fluorescence lifetime measurements [1%] by admin, 2007-07-06 16:34
The fluorescence decay characteristics of 2-aminopurine (2AP) and 2-aminopurine riboside (2APr) have been investigated as a function ...
Two-photon-induced photoisomerization of an azo dye [1%] by admin, 2007-07-06 16:33
The cis-trans photoisomerization of an azo dye was induced by two-photon excitation using a titanium: sapphire femtosecond laser, ...
Quantitative spatial mapping of mixing in microfluidic systems [1%] by admin, 2007-07-06 16:31
Time-resolved fluorescence of 2-aminopurine as a probe of base flipping in m.hhai-DNA complexes [1%] by admin, 2007-07-06 16:30
DNA base flipping is an important mechanism in molecular enzymology,
High-precision FLIM-FRET in fixed and living cells reveals heterogeneity in a simple cfp-yfp fusion protein [1%] by admin, 2007-07-06 16:29
We have used widefield photon-counting FLIM to study FRET in fixed and living cells using control FRET pairs. We have studied ...
Optical tweezer micromanipulation of filamentous fungi [1%] by admin, 2007-07-06 16:23
Optical tweezers have been little used in experimental studies on filamentous fungi. We have built a simple, compact, easy-to-use, safe and robust optical ...
Continuous motion of interference patterns using the angular Doppler effect [1%] by admin, 2007-07-06 16:23
We demonstrate the use of the angular Doppler effect to obtain continuous motion of interference patterns. A small frequency shift between two beams can ...
Study of DNA deformation under flow using optical tweezers [1%] by admin, 2007-07-06 16:22
We have observed a latency time (tau) of the order 10 s in the spermidine-induced condensation of YOYO-1-labelled DNA in flow. Higher flow speeds, longer ...
Condensation of hydrodynamically stretched DNA using single molecule fluorescence imaging and optical tweezers [1%] by admin, 2007-07-06 16:22
The stretching and unwinding of polymers under flow is important for understanding the rheological properties of dilute polymer solutions. Scaling ...
Spherical aberration correction for optical tweezers [1%] by admin, 2007-07-06 16:22
Typical optical tweezers setups use high numerical aperture oil-immersion objectives to trap particles suspended in an aqueous medium. When trapping deep ...
Spectral imaging in a snapshot [1%] by admin, 2007-07-06 16:22
We describe a new filter that simultaneously achieves spectral filtering and image replication to yield a two-dimensional, snapshot spectral imager. ...
Force measurements in colloidal glasses using optical tweezers [1%] by admin, 2007-07-06 16:22
Colloidal systems form the basis of many complex areas of academic and industrial research efforts. As well as contributing to the understanding of many ...
Measuring Fungal Growth Forces with Optical Tweezers [1%] by admin, 2007-07-06 16:22
Optical tweezers are proving themselves useful and powerful tools in many areas of biological research, including fungal cell biology. Here we investigate ...
Hydrodynamics of bacterial suspensions [1%] by admin, 2007-07-06 16:21
Suspensions of motile E. coli bacteria serve as a model system to experimentally study the hydrodynamics of active particle suspensions. Colloidal probe ...
Multiple trap Laguerre-Gaussian holographic optical tweezers using a multiplexed ferroelectric SLM [1%] by admin, 2007-07-06 16:21
A holographic optical tweezers system is constructed using time-shared multiplexing to generate multiple optical traps. Computer generated Fresnel zone ...
Linear and nonlinear microrheology of dense colloidal suspensions [1%] by admin, 2007-07-06 16:21
The length and time scales accessible to optical tweezers make them an ideal tool for the examination of colloidal systems. Embedded high-refractive-index ...
Controlled rotation of optically trapped microscopic particles [1%] by admin, 2007-07-06 16:21
We demonstrate controlled rotation of optically trapped objects in a spiral interference pattern. This pattern is generated by ...
Revolving interference patterns for the rotation of optically trapped particles [1%] by admin, 2007-07-06 16:20
Optically trapped objects are rotated controllably in the interference pattern between a Laguerre-Gaussian (LG) beam and a Gaussian ...
Creation and manipulation of three-dimensional optically trapped structures [1%] by admin, 2007-07-06 15:59
An interferometric pattern between two annular laser beams is used to construct three-dimensional (3D) trapped structures within an ...
Moving interference patterns created using the angular Doppler-effect [1%] by admin, 2007-07-06 15:59
We use the angular Doppler-effect to obtain stable frequency shifts from below one Hertz to hundreds of Hertz in the optical ...
Handedness and azimuthal energy flow of optical vortex beams [1%] by admin, 2007-07-06 15:59
The propagation of optical vortex beams obstructed by a knife edge is investigated. The intensity profile shows a characteristic ...
Controlled simultaneous rotation of multiple optically trapped particles [1%] by admin, 2007-07-06 15:59
We present a method for the controlled alignment or rotation of birefringent particles trapped in multiple optical trap sites of an ...
Single molecule fluorescence imaging and its application to the study of DNA condensation [1%] by admin, 2007-07-09 15:02
Single molecule fluorescence imaging incorporated with optical tweezers and a laminar flow cell has been used to monitor the kinetic process of DNA ...