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E Theofanidou, T J Su, J Arlt, D T Dryden, W J Hossack, J Crain, and W CK Poon (2004)
Condensation of hydrodynamically stretched DNA using single molecule fluorescence imaging and optical tweezers
In: Optical Trapping and Optical Micromanipulation, edited by Dholakia, K. and Spalding, G. C.. SPIE, pages 225–233.
The stretching and unwinding of polymers under flow is important for
understanding the rheological properties of dilute polymer solutions.
Scaling theory based on the blob picture of single polymer chains
predicts several regimes for the overall shape of a hydrodynamically
deformed macromolecule. We studied the shape of a DNA molecule
stretched out by steady uniform flow at different velocities using
optical trapping of single DNA molecules (tethered on polystyrene
beads) and single molecule fluorescence imaging. The results show a
gradual transition from a non-draining regime at low velocities to a
free-draining regime at high velocities, thus verifying the predictions
of the free draining-shell (F-shell) blob model.
Conference on Optical Trapping and Optical Micromanipulation, AUG 02-06, 2004 Denver, CO