Confocal Scanning Laser Microscopy

Scanning Fluorescence Lifetime Imaging

Multiphoton Fluorescence Microscopy

Optical Tweezers Micromanipulation

Local rules for CONFOCAL lab

Confocal Scanning Laser Microscopy (cSLM)

• Nikon Eclipse TE300 inverted microscope
• Ports: front, side, bottom
• Nikon Eclipse TE800 upright microscope
  
• BioRad Radiance 2100 Confocal Scanning Microscope System, comprising:
• Argon-Ion laser providing: 457, 476, 488 & 514 nm
  
• Green Helium-Neon ("GreNe") laser: 543 nm
• Red diode laser: 637 nm
  
• Three PMT detector channels

Scanning Fluorescence Lifetime Imaging (cFLIM)

Time-correlated single-photon counting is provided by

• Becker & Hickl SPC-730 TCSPC PCI-card module

Multiphoton Fluorescence Microscopy

• Coherent Verdi V10 diode-pumped solid state green laser
  
• CW
• 10-Watt
• 532-nm
• Coherent Mira Optima 900-F Modelocked Ti:Sapphire laser
  
• Tuning range: 700-1000 nm (requires N₂ above nm)
  
• Pulse repetition rate: 76 MHz
• Pulse duration < 200 fs
• Output power: 1.3 W from 10-W pump
  
• Horizontal polarization
• TEM₀₀ spatial mode

Optical Tweezers

• Nikon Eclipse TE2000 inverted microscope
• One fixed trap in the centre of the field
• One movable trap
• CCD Camera to monitor and record images and movies
• Software to control the system